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Information and Specification
This document (last updated
5 November 1996) was derived from an original on-line
document written by D.G. Bradley.
The recommendation of a
group of markers for universal use in cattle poses a particular
problem which is not yet encountered in other domestic
species. Cattle are already the focus of much research
activity and large bodies of data pertaining to genetic
variation in different breeds already exist or are in
preparation.
Sadly there is little overlap
in marker choice between laboratories and consequently
joint analyses of data produced will be difficult. The
following 23 markers were agreed at the AIRE project meeting
in Utrecht on 18/19 May 1996 and may be recommended for
use in such work so that joint analysis of future data
will be possible with as much incorporation of previous
work as possible.
The selection of markers
was based on the FAO panel and known usage in groups working
on biodiversity in cattle.
The following criteria
have been employed, but not absolutely, in marker choice.
- Existence of prior population data
- Readability of marker
- Number of alleles
- Suitability for use in an automated sequencer
- Possibility of multiplexing
- Cross-species utility
The panel of markers will
be increased to 30 after agreement at the ISAG meeting
in Tours, France in July 1996.
Further information can
be obtained from Dan
Bradley
The INRA023 and INRA032
primers have been corrected on this page and on the database,
after comparisons in various publications. This was communicated
by Dr I Olsaker at the Norwegian College of Veterinary
Medicine, Oslo, Norway.
Marker
number |
Marker |
Chromosome |
Primer sequences (5' - 3') |
Reference |
| 1 |
INRA063
(D18S5)
|
18 |
ATTTGCACAAGCTAAATCTAACC
AAACCACAGAAATGCTTGGAAG |
14 |
| 2 |
INRA0052
(D12S4) |
12 |
CAATCTGCATGAAGTATAAATAT
CTTCAGGCATACCCTACACC |
13 |
| 3 |
ETH225
(D9S1) |
9 |
GATCACCTTGCCACTATTTCCT
ACATGACAGCCAGCTGCTACT |
12 |
| 4 |
ILSTS005
(D10S25) |
10 |
GGAAGCAATGAAATCTATAGCC
TGTTCTGTGAGTTTGTAAGC |
3 |
| 5 |
HEL51
(D21S15) |
21 |
GCAGGATCACTTGTTAGGGA
AGACGTTAGTGTACATTAAC |
7 |
| 6 |
HEL1
(D15S10) |
15 |
CAACAGCTATTTAACAAGGA
AGGCTACAGTCCATGGGATT |
7 |
| 7 |
INRA035
(D16S11) |
16 |
ATCCTTTGCAGCCTCCACATTG
TTGTGCTTTATGACACTATCCG |
14 |
| 8 |
ETH152
(D5S1) |
5 |
TACTCGTAGGGCAGGCTGCCTG
GAGACCTCAGGGTTGGTGATCAG |
12 |
| 9 |
INRA023
(D3S10) |
3 |
GAGTAGAGCTACAAGATAAACTTC
TAACTACAGGGTGTTAGATGAACTC |
14 |
| 10 |
ETH104
(D5S3) |
5 |
GTTCAGGACTGGCCCTGCTAACA
CCTCCAGCCCACTTTCTCTTCTC |
11 |
| 11 |
HEL9
(D8S4) |
8 |
CCCATTCAGTCTTCAGAGGT
CACATCCATGTTCTCACCAC |
7 |
| 12 |
CSSM663
(D14S31) |
14 |
ACACAAATCCTTTCTGCCAGCTGA
AATTTAATGCACTGAGGAGCTTGG |
1 |
| 13 |
INRA0325
(D11S9) |
11 |
AAACTGTATTCTCTAATAGCTAC
GCAAGACATATCTCCATTCCTTT |
14 |
| 14 |
ETH3
(D19S2) |
19 |
GAACCTGCCTCTCCTGCATTGG
ACTCTGCCTGTGGCCAAGTAGG |
11 |
| 15 |
BM2113
(D2S26) |
2 |
GCTGCCTTCTACCAAATACCC
CTTCCTGAGAGAAGCAACACC |
2 |
| 16 |
BM1824
(D1S34) |
1 |
GAGCAAGGTGTTTTTCCAATC
CATTCTCCAACTGCTTCCTTG |
2 |
| 17 |
HEL135
(D11S15) |
11 |
TAAGGACTTGAGATAAGGAG
CCATCTACCTCCATCTTAAC |
7 |
| 18 |
INRA037
(D10S12) |
11 |
GATCCTGCTTATATTTAACCAC
AAAATTCCATGGAGAGAGAAAC |
14 |
| 19 |
BM1818
(D23S21) |
23 |
AGCTGGGAATATAACCAAAGG
AGTGCTTTCAAGGTCCATGC |
2 |
| 20 |
ILSTS006
(D7S8) |
7 |
TGTCTGTATTTCTGCTGTGG
ACACGGAAGCGATCTAAACG
|
4 |
| 21 |
MM12
(D9S20) |
9 |
CAAGACAGGTGTTTCAATCT
ATCGACTCTGGGGATGATGT |
9 |
| 22 |
CSRM60
(D10S5) |
10 |
AAGATGTGATCCAAGAGAGAGGCA
AGGACCAGATCGTGAAAGGCATAG |
10 |
| 23 |
ETH185
(D17S1) |
17 |
TGCATGGACAGAGCAGCCTGGC
GCACCCCAACGAAAGCTCCCAG |
12 |
| 24 |
HAUT24
(D22S26) |
22 |
CTCTCTGCCTTTGTCCCTGT
AATACACTTTAGGAGAAAAATA |
16 |
| 25 |
HAUT27
(D26S21) |
26 |
TTTTATGTTCATTTTTTGACTGG
AACTGCTGAAATCTCCATCTTA |
16 |
| 26 |
TGLA227
(D18S1) |
18 |
CGAATTCCAAATCTGTTAATTTGCT
ACAGACAGAAACTCAATGAAAGCA |
15 |
| 27 |
TGLA126
(D20S1) |
20 |
CTAATTTAGAATGAGAGAGGCTTCT
TTGGTCTCTATTCTCTGAATATTCC |
15 |
| 28 |
TGLA122
(D21S6) |
21 |
CCCTCCTCCAGGTAAATCAGC
AATCACATGGCAAATAAGTACATAC |
15 |
| 29 |
TGLA53
(D16S3) |
16 |
GCTTTCAGAAATAGTTTGCATTCA
ATCTTCACATGATATTACAGCAGA |
15 |
| 30 |
SPS115
(D15) |
15 |
AAAGTGACACAACAGCTTCTCCAG
AACGAGTGTCCTAGTTTGGCTGTG |
17 |
1, 2, 3, 4, 5 - the genetic distance between the marker
pairs on the same chromosomes is not known.
The following markers were
recommended in ISAG, Tours for parentage testing (three
multiplexes):
-
BM1824
BM2113
SPS115
-
ETH3
ETH10
ETH225
-
TGLA122
TGLA126
TGLA227
-
Barendse
W., Armitage S.M. et al. (1994).
A genetic linkage map of the bovine genome.
Nature Genetics 6 227-235.
-
Bishop
M.D. and Kappes S.M. et al. (1994).
A genetic linkage map for cattle.
Genetics 136 619-639.
-
Brezinsky
L.S., Kemp J. And Teale A.J. (1993a).
ILSTS005: a polymorphic bovine microsatellite.
Animal Genetics 24 73.
-
Brezinsky
L.S., Kemp J. And Teale A.J. (1993b).
ILSTS006: a polymorphic bovine microsatellite.
Animal Genetics 24 73.
-
Brezinsky
L.S., Kemp J. And Teale A.J. (1993c).
Five polymorphic bovine microsatellites (ILSTS010-014).
Animal Genetics 24 75-76.
-
Fries
R., Eggen A. and Womack J.E. (1993).
The bovine genome map.
Mammalian Genome 4 405-428.
-
Kaukinen J, Varvio SL.
(1993).
Eight polymorphic bovine microsatellites.
Animal Genetics 24 148.
-
Kemp
S.J., Hishida O. et al. (1995).
A panel of polymorphic bovine, ovine and caprine microsatellite
markers.
Animal Genetics 26 299-306.
-
Mommens
G.W., Coppieters A. et al. (1994).
Dinucleotide repeat polymorphism at the bovine MM12E6
and MM8D3 loci.
Animal Genetics 25 368.
-
Moore
S.S., Byrne K. et al. (1994).
Characterisation of 65 bovine microsatellites.
Mammalian Genome 5 84-90.
-
Solinas
Toldo S., Fries R. et al. (1993).
Physically mapped, cosmid-derived microsatellite markers
as anchor loci on bovine chromosomes.
Mammalian Genome 4 720-727.
-
Steffen
P., Eggen A. et al. (1993).
Isolation and mapping of polymorphic microsatellites in
cattle.
Animal Genetics 24 121-124.
-
Vaiman
D., Osta D. et al. (1992).
Characterisation of five new bovine microsatellite repeats.
Animal Genetics 23 537.
-
Vaiman
D., Mercier D. et al. (1994).
A set of 99 cattle microsatellites: characterisation,
synteny mapping, and polymorphism.
Mammalian Genome 5 288-297.
-
Georges,
M. and Massey, J.M.. (1992).
Polymorphic DNA markers in Bovidae.
Patent WO 92/13102 (1992).
-
Thieven
U., Solinos-Toldo S. et al. (1997).
Polymorphic CA-microsatellites for the integration of
the bovine genetic and physical map.
Mammalian Genome 8 52-55.
-
Moore,
S.S. and Byrne, K.. (1993).
Dinucleotide polymorphism at the bovine calmodulin independent
adenylcyclase locus.
Animal Genetics 24 150.
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