bola class II haplotypes

BoLA Nomenclature

International Society for Animal Genetics

BoLA Class II haplotypes

These data are extracted from the Fifth BoLA workshop report where a detailed discussion of the haplotypes and their derivation can be found.

Click here to see haplotypes sorted by serological typing

Class IIa haplotypes sorted by DRB3 PCR-RFLP.

DH^a	Class II serology	DRB IEF^b	DRB3 PCR- RFLP	DRB3 MS PCR	DRB2 TM RFLP	DRB RFLP	DQA RFLP	DQB RFLP	DQB TM RFLP	Breed (no)^c
1A	ND^d	9	1	189	11	5A	5	5	Negⁱ	BFxHE(1)
3A	Dw1;Dc6	5	3	195	21	10	10	10	14	BF(4),DB^j(2)
3B	Dc6	12	3	207	21	10	7F	7E	53	BF(1)
7A	Dw2	2	7	193	32	2A	2	2	44	BF(1),DB^j(7), HF(1)BfxHE(1)
7A	Dw2	2	7	193	32	2B	2	2	44	JE^k(1)
8A	Dw1;Dc12	6	8	207	46	12B	12	12	213	BF(4),DB^j(5)
9A	Dc13	U19	9	185	26	3F	3A	3B	43	JE^k(1)
9B	ND	ND	9	185	23	3C	3B	3B	43	BS^j(1)
10A	ND	7	10	165	44	14	14	14	46	JE(1)
10B	ND^e	6	10	193	25	11A	14	14	46	AyxLM(1)
10C	Dw1,8	6	10	193	25	11A	11B	11A	11	BS(1),DR^j(2)
11A	Dc13,20	1	11	187	11	3D	3A	3A	43	BF(3),HF^l(1)
11A	Dc13,20	1	11	187	11	3E	3A	3A	43	BfxAY(1)
11B	Dc13,20	1	11	187	11	3E/G^g	3B	3B	43	DR^k(1)
11C	Dw1	6	11	195	25	ND	9A	9A	13	DR^k(1)
12A	Dc5^e	6	12	179	31	13C	13B	13B	11	AyxLM(1)
12B	Dc5	6	12	185	21	7E	7C	7E	53	BfxHE(1)
15A	Dw3	U17	15	167	21	1F	1D	1	45	DR^j,l(5)
15B	Dw3	6	15	183	13	1E	1E	1	45	BF(2)
15C	ND	U18	15	185	11	3F	3B	3B	43	JE^j(4)
16A	Dw1,8	5	16	193	25	11A	11A	11C	13	DB^j(7),BFxAY(1)
17A	Dw1	ND	17	179	21	11E	11D	11E	11	JE^j(1)
18A	Dw1,4;Dc7	4	18	183	46	5B	5	5	Neg	DB^k(2),BFxHE(1)
18A	Dw1,4;Dc7	4	18	183	46	5C	5	5	Neg	BF(3)
20A	Dw3	U21	20	173	46	1G	1D	1	45	JE^j(1)
21A	ND	4	21	187	21	8A	8	8	46	JE(1)
21A	ND	4	21	187	21	8C	8	8	46	BF(1)
22A	Dc13	11	22	165	24	3I	3C	3C	33	BF(3),DB^j(2)
22B	Dw1,Dc9,12	7	22	175^f	25	11A	9B	9B	42	DB^j(3)
22C	Dw1,8;Dc9	7	22	187	25	11A	11A	11A	12	BF(2),DB^j(2)
22E	Dw1;Dc9	7	22	187	25	11A	11F	11C	13	DR^j(3)
22F	Dc9	7	22	187	25	4A	3A	4	43	BF(1)
22G	ND	11	22	191	11	3H	3B	3C	33	BS(1)
23A	Dw3	10	23	157	11	7D^h	7D	7A	45	HF(2)
24A	Dw3	3	24	185	25	1A	1A	1	45	BF(5),DB^k(3),HF(1)
27A	Dw3;Dc11	7	27	171	25	1B	1B	1	45	DR^k(3)
28A	Dw3	8	28	163	11	7A	7A	7A	45	BF(4),BS^k(1),DB^j(3)
31A	Dw1,4	2	31	181	25	5D	5	5	Neg	DB^k(4)

^aClass IIa haplotype (DH) reference numbers.
^b Number by themselves are DRBF types, numbers preceded by a U are UDF types.
^cBreeds: AY=Ayrshire; BF=British Friesian; BS=Brown Swiss; DB=Danish Black Pied; DR=Danish Red; HE=Hereford; HF=Holstein Friesian; JE=Jersey; LM=Limousin. Number of animals with haplotype shown in brackets. Results for 59 animals, WK5-47 was omitted because her haplotypes could not be defined.
^dNot determined.
^eHaplotype may express Dw1 and/or Dw8.
^f Cow, WK5-45, was assigned DRB3-MS*179 rather than DRB3-MS*175 (see results).
^gURB and MLI assigned different patterns to this animal.
^h WK5-59 was assigned a 6.4 kb TaqI fragment by both MLI and URB. Consequently, this animal apparently had the DRB*3F rather than the DRB*7D pattern. However, as the two animals were of the same breed and had the same class I serotype the data was not deemed conclusive enough to split this haplotype.
ⁱ Negative. Missing or inconsistent results due to weak hybridization.
^j Confirmed by segregation in this breed.
^k Occurred opposite a segregating haplotype in this breed.
^l Includes one class IIa homozygote.

Class IIa haplotypes sorted by serological specificities..

DH^a	Class II serology^b			DRB IEF	DRB3 PCR- RFLP	DRB3 MS PCR	DRB2 TM RFLP	DRB RFLP	DQA RFLP	DQB RFLP	DQB TM RFLP	Number^c
3B			Dc6	12	3	207	21	10	7F	7E	53	1
3A	Dw1		Dc6	5	3	195	21	10	10	10	14	6^gh
8A	Dw1			6	8	207	46	12B	12	12	213	8
11C	Dw1			6	11	195	25	ND	9A	9A	13	1^gh
17A	Dw1			ND^d	17	179	21	11E	11D	11E	11	1^g
31A	Dw1	Dw4		2	31	181	25	5D	5	5	Neg^e	4
18A	Dw1	Dw4	Dc7	4	18	183	46	5B	5	5	Neg	3^gh
18A	Dw1	Dw4	Dc7	4	18	183	46	5C	5	5	Neg	2
10C	Dw1	Dw8		6	10	193	25	11A	11B	11A	11	3
16A	Dw1	Dw8		5	16	193	25	11A	11A	11C	13	8^gh
22C	Dw1	Dw8	Dc9	7	22	187	25	11A	11A	11A	12	4
22E	Dw1		Dc9	7	22	187	25	11A	11F	11C	13	3
22B	Dw1		Dc9	7	22	175	25	11A	9B	9B	42	3^g
22F			Dc9	7	22	187	25	4A	3A	4	43	1^h
7A	Dw2			2	7	193	32	2A	2	2	44	10^gh
7A	Dw2			2	7	193	32	2B	2	2	44	1
27A	Dw3		Dc11	7	27	171	25	1B	1B	1	45	3^h
24A	Dw3			3	24	185	25	1A	1A	1	45	9^gh
15A	Dw3			U17	15	167	21	1F	1D	1	45	5
20A	Dw3			U21	20	173	46	1G	1D	1	45	1
15B	Dw3			6	15	183	13	1E	1E	1	45	2
28A	Dw3			8	28	163	11	7A	7A	7A	45	7^gh
23A	Dw3			10	23	157	11	7D	7D	7A	45	2^g
12A^f			Dc5	6	12	179	31	13C	13B	13B	11	1^g
12B			Dc5	6	12	185	21	7E	7C	7E	53	1^g
9A	Dc13			U19	9	185	26	3F	3A	3B	43	1
22A	Dc13			11	22	165	24	3I	3C	3C	33	5
11A	Dc13		Dc20	1	11	187	11	3E	3A	3A	43	3
11A	Dc13		Dc20	1	11	187	11	3D	3A	3A	43	1
11B	Dc13		Dc20	1	11	187	11	3E/G	3B	3B	43	1

^aClass IIa haplotype (DH) reference numbers.
^bSpecificities that appear to correspond to DR or DQ molecular motifs are indicated by fine and bold boxes, respectively. Dc12 which reacts with DH8A and DH22B is not shown because although it is probably a DQ specificity it is not associated with a molecular motif.
^cNumber of animals with haplotype for which serological data was available.
^dNot determined.
^eNegative. Missing or inconsistent results due to weak hybridization.
^fHaplotype may express Dw1 and/or Dw8.
^g Supported by data from Fourth BoLA Workshop ( Bernoco et al., 1991).
^h Supported by data from Davies et al., 1992a.